site stats

Cdc13 phosphorylation

WebApr 18, 2012 · Cdc13 is a single-strand telomeric DNA-binding protein that positively regulates yeast telomere replication by recruiting telomerase to chromosome termini through a site on Cdc13 that is eliminated by the cdc13-2 mutation. WebJun 26, 2024 · Indeed, the transition from the Cdc13-Est1 interaction to the CST complex formation seems to be regulated in part by sequential phosphorylation of Cdc13 and Stn1 by Cdk1 [89,90] (Figure 3D).

Two Distinct Cdc2 Pools Regulate Cell Cycle Progression and the

WebFor example, phosphorylation of S314 was significantly higher in the G2/M compared to the G1 phase and in WT versus mutant Cdc13, and a S314D mutation negatively affected telomere length. Our findings provide new targets in a key telomerase regulatory … WebJul 1, 2015 · While all four H-forms bind to cyclin B (Cdc13 Sp ), which implies that they carry the T167 phosphorylation, only two H-forms (He + Hf) are the target of the DNA damage checkpoint as the Y15 modification is restricted to them. The most extensively phosphorylated form, Hf, may also be modified at T14 as its abundance strongly declines … integris employee healthstream https://puretechnologysolution.com

The Hydrophobic Patch Directs Cyclin B to Centrosomes to …

WebMar 9, 2024 · We observed that phosphorylation by Cdc13 HPM-CDK displayed a relationship with cellular compartment, as SPB and cytoplasmic substrates cannot be phosphorylated to wild-type levels, whereas nuclear and nuclear-envelope-localized substrates are equally phosphorylated by Cdc13 WT-CDK and Cdc13 HPM-CDK (Figure … Web(E) The expression of cdc13+ in WT (JY333) and ecls∆ (JY333 ecl1∆ ecl2∆ ecl3∆) cells during sulfur depletion (n = 3). (F) Levels of Cdc13 and the Cdc2-Tyr15 phosphorylation under sulfur depletion (n = 3). (G) Cells were collected 1 day after transfer to sulfur-depleted medium, and levels of Cdc13 were WebJan 9, 2009 · Cdc13 Phosphorylation Affects Telomerase-Dependent Telomere Maintenance To further understand the functional significance of Cdk1-dependent phosphorylation of Cdc13, we tested whether the phosphorylation of Cdc13 threonine 308 is involved in telomerase-dependent or telomerase-independent telomere … integris employee health

SUMOylation regulates telomere length homeostasis by …

Category:Rapid Cdc13 turnover and telomere length homeostasis …

Tags:Cdc13 phosphorylation

Cdc13 phosphorylation

Cdk1-Dependent Phosphorylation of Cdc13 Coordinates …

WebIn this last situation, Cdc2-Tyr15 phosphorylation could easily be detected in these HU-blocked cells despite the absence of Cdc13, showing that Cdc2/cyclin complex(es) other than Cdc2/Cdc13 are ... WebThese data suggest that Cdc13 threonine 308 is phosphorylated by Cdk1 in vivo. Treatment of the asynchronous yeast culture with 1-NM-PP1 also results in some enrichment of yeast in G1 phase of the cell cycle, as shown by FACS analysis of cellular DNA content (Figure 3D, compare 0 min to 15 and 30 min time points).

Cdc13 phosphorylation

Did you know?

WebCdc13p is the single essential cyclin in Schizosaccharomyces pombe and a member of the cyclin B family. Cdc13p abundance rises during G 2 -phase and falls as cells progress through mitosis and G 1. Cdc13p degradation, …

WebMar 9, 2024 · Our experiments suggest that the hydrophobic patch of mitotic cyclins contributes to CDK substrate selection by directing the localization of Cdc13-CDK to … WebCdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 …

WebJan 9, 2009 · Here we show that Cdk1-dependent phosphorylation of Cdc13 is essential for efficient recruitment of the yeast telomerase complex to telomeres by favoring the interaction of Cdc13 with Est1 rather than … WebAug 25, 2010 · Moreover, the phosphorylation of Bir1 was reduced in the cdc13-M7 mutant in prometaphase . These results indicate that Bir1 is phosphorylated at the CDK consensus sites in vivo , most likely by ...

WebPhosphorylation of Cdc13 by Cdk1 promotes the interaction between Cdc13 and Est1 and hence telomere elongation. Here, we show that Cdk1 also phosphorylates Stn1 at threonine 223 and serine 250 both in vitro and in vivo, and these phosphorylation events are essential for the stability of the CST complexes at the telomeres.

WebBackground The fission yeast Schizosaccharomyces pombe is widely-used as a model organism for the study of a broad range of eukaryotic cellular processes such as cell cycle, genome stability and cell morphology. Despite the availability of extensive integris employee loginWebNov 12, 2014 · As was mentioned above, Tel1/Mec1mediated Cdc13 phosphorylation promotes the Cdc13-Est1 interaction [82, 83]. In turn, this effect is counteracted by the PP2A phosphatase and the Aurora kinase [83 integris employee health numberWebCdc13 phosphorylation quantitative proteomics Supporting Information Histogram of the ratio of 23-DFEGYPSK-30 to 567-LELNEGFK-574, and annotated CID tandem mass spectra. This material is available free of charge via the Internet at http://pubs.acs.org. pr300408v_si_001.pdf (569.31 kb) pr300408v_si_002.pdf (57.49 kb) Terms & Conditions integris employee health hoursWebJan 9, 2009 · Cdc13 Phosphorylation Affects Telomerase-Dependent Telomere Maintenance To further understand the functional significance of Cdk1-dependent … integris employee assistance programWebOct 3, 2011 · Cdc13 is thought to be a Tel1/Mec1 target as both kinases can phosphorylate N-terminal fragments of Cdc13 in vitro. Moreover, simultaneous mutation of two of the … integris emergency room oklahoma cityWebJun 29, 2024 · The tightly regulated timing of mitosis in S. pombe occurs via the reciprocal activities of Cdc25 and Wee1 on Cdc2 inhibitory phosphorylation. Wee1 negatively regulates Cdc2 by phosphorylation of tyrosine residue 15 (Tyr15), and this is counteracted by the phosphatase activity of Cdc25 [1,2,3].Cells must advance or delay mitosis under … integris employee scrubsWebApr 9, 2009 · One apparent hypothesis for the CDK1 -dependent Cdc13 phosphorylation is that Cdc13 may be directly phosphorylated by Cdk1. The CDK family of kinases has a … integris employee health okc