WebApr 18, 2012 · Cdc13 is a single-strand telomeric DNA-binding protein that positively regulates yeast telomere replication by recruiting telomerase to chromosome termini through a site on Cdc13 that is eliminated by the cdc13-2 mutation. WebJun 26, 2024 · Indeed, the transition from the Cdc13-Est1 interaction to the CST complex formation seems to be regulated in part by sequential phosphorylation of Cdc13 and Stn1 by Cdk1 [89,90] (Figure 3D).
Two Distinct Cdc2 Pools Regulate Cell Cycle Progression and the
WebFor example, phosphorylation of S314 was significantly higher in the G2/M compared to the G1 phase and in WT versus mutant Cdc13, and a S314D mutation negatively affected telomere length. Our findings provide new targets in a key telomerase regulatory … WebJul 1, 2015 · While all four H-forms bind to cyclin B (Cdc13 Sp ), which implies that they carry the T167 phosphorylation, only two H-forms (He + Hf) are the target of the DNA damage checkpoint as the Y15 modification is restricted to them. The most extensively phosphorylated form, Hf, may also be modified at T14 as its abundance strongly declines … integris employee healthstream
The Hydrophobic Patch Directs Cyclin B to Centrosomes to …
WebMar 9, 2024 · We observed that phosphorylation by Cdc13 HPM-CDK displayed a relationship with cellular compartment, as SPB and cytoplasmic substrates cannot be phosphorylated to wild-type levels, whereas nuclear and nuclear-envelope-localized substrates are equally phosphorylated by Cdc13 WT-CDK and Cdc13 HPM-CDK (Figure … Web(E) The expression of cdc13+ in WT (JY333) and ecls∆ (JY333 ecl1∆ ecl2∆ ecl3∆) cells during sulfur depletion (n = 3). (F) Levels of Cdc13 and the Cdc2-Tyr15 phosphorylation under sulfur depletion (n = 3). (G) Cells were collected 1 day after transfer to sulfur-depleted medium, and levels of Cdc13 were WebJan 9, 2009 · Cdc13 Phosphorylation Affects Telomerase-Dependent Telomere Maintenance To further understand the functional significance of Cdk1-dependent phosphorylation of Cdc13, we tested whether the phosphorylation of Cdc13 threonine 308 is involved in telomerase-dependent or telomerase-independent telomere … integris employee health